Syngas Biofuels Energy, Inc.
Additional Products and Services
                                                     Bench-Scale Multi-Vessel Fermentor
  There is no need to emphasize importance of testing recombinant clones under conditions similar to industrial fermentations - in flexible multi-vessel bioreactors with bench-type
footprint but still with the whole array of parameters to adjust for specific applications. Scientific community had been set back for decades by the need to pay for individual
fermentation vessels above $10,000 each and also have overhead expenses just to have multiple units running in extra size cGLP space.
Syngas Biofuels Energy, Inc. offers the
first in the world breakthrough in bioreactor recombinant clone testing process, which saves up to ~$5,000 per individual vessel, in addition to cutting down expenses for utilities,
facility and personnel by about 65 %.

  Syngas Biofuels Energy, Inc. bench-scale 36 vessel bench scale multi-fermentor is just $220,000.00 FOB Houston, or just $6,111.00 per individual vessel (total 36 individual
vessels). It is essential for express analysis of recombinant clones in continuous fermentation conditions nearly those anticipated during scaling up. Thirty six independent
autoclavable vessels with hermetically sealable screw caps with multiple ports for sensors, temperature and pH control and proprietary cell density meter sensor offering only viable
cell count data as opposite to cell density curves based on OD
600 and cell dry weight, for improved accuracy of recombinant control.  With two Bench-Scale Multi-Fermentors, one
researcher would be able to analyse up to 720 clones on a monthly basis at the turn around time for each clone 3 days in batch mode or up to 72 clones for up to 30 days in
continuous mode. We offer Peltier coolers of went gas tubular circuitry to eliminate water losses during gas blend fermentation.  This si the real breakthrough compared with clone
growth in just sealed bottles - cut down clone analysis time for acetogens to just 48 hours.
                                                
               Each Vessel of Bench-Scale Multi-Fermentor has:
  • 0 - 50 ml max liquid volume
  • Controlled stirring speed 5 – 3200 rpm
  • Temperature control from ambient to 56 degrees C
  • Water vapor cold trap assembly (-10 Degree C to +10 Degree C) with vent gas moisture condenser
  • pH control with acid and alkali ports
  • Living cell density sensor
  • Inoculation, feed, drain ports
  • Foaming sensor

Multi-Fermentor has
  •        Size: 48" x 48" x 36”
  •        Weight: 210 lbs (300 lbs in transportation container).
Each Bench-Scale Multi-Fermentor is sold with five year limited warranty.  No lease to buy option is available at this time

                                                                                                  MT254UVnator

The breakthrough in time- and cost-efficient generation of multiple mutations in anaerobic organisms was achieved with introduction of the unique  MT254UVnator rendering 18
Joules of sharp 254 nm UV-C energy right underneath the apparatus to treat cell suspensions in standard 100 mm Petri dishes.  Each MT254UVnator is only $4,400.00 FOB
Houston and comes with five years limited warranty. We suggest you do not try to use schematic design shown below since you will never achieve results we do due to our
proprietary details and "know how" not shown specifically to avoid illegal copying of our proprietary design - buy and enjoy your fast and efficient mutagenesis.
Syngas Biofuels Energy, Inc. offers   R & D service opportunities on a project basis starting from $1M
per project contract. Average contract is $5M with flexibility to vary the final price based on customer
satisfaction approach  

Possible levels of contracts:

•      
  turnkey end point – customers get entire project done per their specifications with no life cycle design and control option;
•        
life cycle design along with turnkey level of performance with indications on where the bottlenecks in revenue generation will be in the future based on comprehensive
market evolution research and analysis;
•        
life cycle control over the industry and market trends with generation of additional revenue opportunities on as needed/deem appropriate basis over the years – the
continuation is free and means life-long servicing that particular customer.

Particular topics of interest include but are not limited to the flowing areas of our top-notch in the industry expertise:

1.        Biologically enhanced petroleum recovery – biological mapping of your particular oil fields and depositing your oil recovering microorganisms for safe storage. Scaling up at
your request and participation in restoration of mature well productivity. Bioremediation of environment polluted due to well biological stimulation and other EPA reportable spills not
related to enhanced petroleum recovery.
2.        Corrosion mitigation strategy for your proprietary pipeline systems
3.        Ligno-cellulosic biomass conversion to fermentable carbohydrates with fermentable carbohydrates recovery rate up to 90 % as per original dry weight – lignin becomes
fermentable as well due to proprietary physical pre-treatment technology.
4.        Bioremediation of your land with proprietary soil recovery technology.
5.        Manure management at your farmland.  Decrease of manure viscosity to liquid level in manure ponds using proprietary strains in manure ponds to increase volume of liquid
manure for use as fertilizer.
6.        Due diligence, control measures to prevent industrial espionage as per your technology and proprietary IP
7.        Express tools to detect your specific biological object (biocatalyst, products containing biologics) using just a single molecule of nucleic acid detection procedure for less
then $1.00 and time below 25 min with no lab needed.  Express tracing of stolen strains with evidence production for litigation process.
8.        Expert witness services for your biotechnology or pharma industry needs to fight industrial espionage and unlawful use of your proprietary biotechnology.
9.        Silage starters, starter cultures for dairy, meat and vegetable fermentations.  Starters will be lyophilized with over 30 years long shelf life with 90% viability upon that time.
10.      Proprietary powerful anti-oxidants and complex probiotics for human and animal use.
11.      Polyhydroxyalcanoates (PHAs) as components of biodegradable plastics: technology to manufacture PHAs from carbon dioxide used as feedstock at disruptive
manufacturing costs.  No problems with PHA recovery from cells with even eight to twelve carbons long PHA side chains – proprietary biocatalyst design to avoid PHA cell
packaging reducing product recovery. PHA recovery rates up to 95% for long side chain biopolymers.
12.      Any sort of biocatalyst development with near theoretical performance using our proprietary genome tailoring technology. Theft-proof biocatalysts
with no need for patent protection.
                                                         Electroporation / Electrofusion References

1.        Gak E, Tyurin M, Kiriukhin M (2014) Genome tailoring powered production of isobutanol in continuous CO2/H2 blend fermentation using engineered acetogen biocatalyst. J
Ind Microbiol Biotechnol. 41(5):763-781.
2.        Kiriukhin M, Tyurin M. (2013) Mevalonate production by engineered acetogen biocatalyst during continuous fermentation of syngas or CO
2/H2 blend. Bioprocess and
Biosystems Engineering - DOI: 10.1007/s00449-013-0991-6.
3.        Tyurin M. (2013) (Invited) Reversal of global warming using $3 trillion market force: chemicals and fuels produced directly and selectively in continuous fermentations of gas
blends comprising CO and CO
2. In: Environmental Aspects of Global warming. Nova Science Publications Press. – New Developments in Global Warming Research. Eds: Carter B.
Keyes and Olivia C. Lucero.
https://www.novapublishers.com/catalog/product_info.php?products_id=46570
4.        Gak E, Tyurin M, Kiriukhin M (2014) UV-induced mutagenesis in acetogens: resistance to methanol, ethanol, acetone, or n-butanol in recombinants with reduced genomes
during continuous CO
2 / H2 gas blend fermentation. World Journal of Microbiology and Biotechnology. DOI: 10.1007/s11274-013-1579-7.
5.        Tyurin M, Kiriukhin M (2013). 2,3-Butanediol production by engineered acetogen biocatalyst during continuous fermentation of syngas or CO
2/H2 blend. Appl Biochem
Biotechnol. 170 (6): 1503-1524. DOI: 10.1007/s12010-013-0285-0.
6.        Tyurin M, Kiriukhin M. (2013). Selective methanol or formate production during continuous CO
2 fermentation by the acetogen biocatalysts engineered via integration of
synthetic pathways using
Tn7-tool. World Journal of Microbiology and Biotechnology. 29 (9)1611-1623. DOI: 10.1007/s11274-013-1324-2.
7.        Tyurin M. (2013). Gene replacement and elimination using AlphaRed- and FLP-based tool to re-direct carbon flux in acetogen biocatalyst during continuous CO
2/H2 blend
fermentation. Journal of Industrial Microbiology & Biotechnology. 40 (7):749-758. DOI: 10.1007/s10295-013-1279-1.
8.        Berzin V, Kiriukhin M, Tyurin M. (2012) Selective production of acetone during continuous synthesis gas fermentation by engineered biocatalyst
Clostridium sp. MAceT113.
Letters of Appl Microbiol. DOI10.1111/j.1472-765X.2012.03272.x.
9.        Tyurin M, Kiriukhin M. (2013). Expression of amplified synthetic ethanol pathway integrated using
Tn7-tool and powered at the expense of eliminated pta, ack, spo0A and
s
po0J during continuous syngas or CO2 /H2 blend fermentation. J Appl Microbiol.  114(4):1033-45. doi: 10.1111/jam.12123
10.       Tyurin M, Kiryukhin M, Berzin V. (2012) Electrofusion of untreated cells of the newly isolated acetogen Clostridium sp. MT351 with integrated in the chromosome erm(B) or
cat leading to the combined presence of these antibiotic resistance genes in the chromosome of the electrofusion products. Journal of Biotech Research. 4:1-12.
11.        Berzin V, Kiriukhin M, Tyurin M. (2013)
Cre-lox66/lox71-based elimination of phosphotransacetylase or acetaldehyde dehydrogenase shifted carbon flux in acetogen
rendering selective overproduction of ethanol or acetate. Appl Biochem Biotechnol. 195(3):181-8. DOI: 10.1007/s12010-012-9864-8
12.        Berzin V, Kiriukhin M, Tyurin M. (2013) Selective n-butanol production by
Clostridium sp. MTButOH1365 during continuous synthesis gas fermentation due to expression
of synthetic thiolase, 3-hydroxy butyryl-CoA dehydrogenase, crotonase, butyryl-CoA dehydrogenase, butyraldehyde dehydrogenase and NAD-dependent butanol
dehydrogenase. Appl Biochem Biotechnol. 169(3), 950-959. DOI: 10.1007/s12010-012-0060-7
13.        Berzin V, Kiriukhin M, Tyurin M. (2013) “Curing” of plasmid DNA in acetogen using microwave or applying an electric pulse improves cell growth and metabolite production
as compared to the plasmid-harboring strain. Arch. Microbiol. 195(3), 181-188. DOI 10.1007/s00203-012-0862-6
14.        Berzin V, Tyurin M. (2012). Acetogen biocatalyst
Clostridium sp. MTEtOH871 engineered with our proprietary electrotransformation technology and equipment: continuous
synthesis gas fermentation for selective ethanol production. Journal of Biotech Research. 4:54-64.
15.        Berzin V, Kiriukhin M., Tyurin M. (2012) Elimination of acetate production to improve ethanol yield during continuous synhesis gas fermentation by engineered biocatalyst
Clostridium sp. MTEtOH550. Appl Biochem Biotechnol. 167 (2):338-347. DOI 10.1007/s12010-012-9697-5.
16.        Shaw AJ, Tyurin M, Podkaminer K, Thorne P, Bardsley J, Rogers S, Hogsett D, Lynd LR (2006). Metabolic engineering of the xylose-utilizing thermophile. Biomass, 1-22
17.        Tyurin MV, Lynd LR, Wiegel J. (2006) Gene transfer systems for obligately anaerobic thermophilic bacteria. Extreophilic Microorganisms. Methods in Microbiology. Eds:
Fred Rainey and Aharon Oren. Elsevier. 35: 307-328. (Invited)
18.        Shaw J, Martin L, Desai S, Tyurin M, Lynd L (2005). Metabolic engineering of the xylose utilizing thermophile Thermoanaerobacterium saccharolyticum JW/SL-YS485 for
ethanol production. AICHE Annual Meeting. November 1st, 2005
19.        Tyurin MV, Sullivan CR, and Lynd LR. (2005) Role of spontaneous current oscillations during high-efficiency electrotransformation of thermophilic anaerobes. Appl Envir
Microbiol. 71:8069—8076
20.        Tyurin M, Desai S, Mielenz J, Lynd L. (2004) Transformation & metabolic engineering of
Clostridium thermocellum. Clostridium VIII. Edinburgh, Scotland. July 3, 2004
21.        Lynd LR, Desai SD, Tyurin MV, Zhang Y, Liu Y, Mielenz JR, Dale BE (2004) Genetic system development, metabolic engineering, bioenergetics and kinetics relevant to
ethanol production using thermophilic bacteria. 26th Symposium on Biotechnology for Fuels and Chemicals. Chatanooga, Tennessee, May 10, 2004
22.        Lynd LR, Zhang Y, Liu Y, Tyurin M, Desai S, Mielenz J (2004) Advances in physiological understanding of thermophilic saccharolytic anaerobic bacteria relevant to their
use as industrial microorganisms. Metabolic Engineering V-th Meeting, Palo Alto, Sept. 2004
23.        Tyurin MV, Desai SG, Lynd LR (2004) Electrotransformation of Clostridium thermocellum. Appl Envir Microbiol. 70(2): 883-890
24.        Tyurin MV, Desai S, Lynd LR (2003) Development and application of genetic systems for anaerobic, thermophilic, ethanol-producing bacteria. Abstract. 25th Symposium
on Biotechnology for Fuels and Chemicals. National Renewable Energy Laboratory. Oak Ridge, TN. November 2003. Poster Presentation 2-37
25.        Tyurin MV, Desai SG, Lynd LR (2003) Electrotransformation of
Clostridium thermocellum.  Abstract. The 103rd ASM General Meeting, 2003.  
26.        Lynd LR, Zhang Y, Fan Z, Desai S, Tyurin M, La Grange D, Gundllapalli S, Cordero-Otero R, van Zyl W, Pretorius I, Weimer P. The microbial cellulose utilization paradigm:
fundamentals & implications for consolidated bioprocessing.  24th Symposium on Biotechnology for Fuels and Chemicals. Gatlinburg, TN. April 29, 2002 Biotechnology for fuels
and chemicals : proceedings of the Twenty-Fourth Symposium on Biotechnology for Fuels and Chemicals, held April 28-May 1, 2002, in Gatlinburg, TN Totowa, N.J. : Humana
Press, ©2003. Applied biochemistry and biotechnology, v. 105-108.
27.        Tyurin MV, Schneider MF (2001) Electrotransformation of skeletal muscle fibers with high voltage radio frequency modulated short pulses. Biophysical Society 45th
Annual Meeting. February 17-21, 2001. Boston, MA// Biophysical Journal. 80:146a-147a
28.        Tyurin MV, Schneider FM (2000). Electrotransformation of single muscle fibers with radio-frequency bipolar pulses.// Muscle Molecular Biology and Gene Transfer.
Interdisciplinary Program in Muscle Biology: Sixth Annual Mini-Retreat. UMAB, Baltimore, MD 21201, April 07, 2000. p.7.
29.        Tyurin MV, Padda RS, Huang K-X, Wardwell S, Caprette D, Bennett GN (2000) Electrotransformation of
Clostridium acetobutylicum ATCC 824 using high-voltage radio
frequency modulated square pulses // J Appl Microbiol. 88(2):220-227
30.        Tyurin MV, Padda RS, Huang K-X, Wardwell S, Caprette D, Bennett GN (1999) Improved electrotransformation method for Clostridium acetobutylicum ATCC 824 // ASM
1999 Annual Congress - June 03, 1999
31.        Tiurin MV, Voroshilova EB, Rostova YuG, Oparina NYu, Goussyatiner MM (1998) Electric response of internal membrane structures of corynebacteria during
electrotransformation. Mikrobiologiia 67(3):338-344  
32.        Bulina TI, Terekhova LP, Tiurin MV (1998) [Use of electrical impulses for selective isolation of actinomycetes from soil] Mikrobiologiia 67(4):556-560
33.        Tyurin MV (1997): Possible electric response of inner cell membranes during electroporation - experimental model. 17-th International Congress on Biochemistry and
Molecular Biology (San Francisco, August 24 - 29, 1997) // The FASEB Journal. 11 (9): A 1089
34.        Tyurin MV, Doroshenko VG, Oparina NYu (1997) Electrofusion of Escherichia coli cells. Membr Cell Biol. 11 (1): 121 – 129
35.        Terekhova LP, Bulina TA, Alferova IV, Tyurin MV (1996) Use of method based on physical factors for isolation of actinomycetes // Abstract. 8-th International. Congress
for Culture Collections. Veldhoven, The Netherlands, August 25 - 29, 1996. P. 449
36.        Tyurin MV, Livshits VA (1996) Electrotransformation of untreated streptomyces cells// Membrane and Cell Biology. 10 (3): 303 – 309
37.        Tyurin MV, Oparina NYu, Livshits VA (1996) Method of cell preparation and influence of pulse form on electrotransformation efficiency of Gram-positive rods and cocci //
Mikrobiologiya (Russ.) 65 (5): 668 – 674
38.        Tyurin MV, Starodubtseva LI, Kudryavtseva EV, Livshits VA, Vojejkova TA (1995) Electrotransformation of germinating spores of
Streptomyces spp. // BioTechnology
Techniques 9 (10): 737 – 740
39.        Tyurin MV, Livshits VA (1995) Methods for plasmid DNA transfer to
Lactobacillus buchneri: natural competence, conjugative mobilization, and electrotransformation //
Membrane and Cell Biology. 9: 57 – 68
40.        Tyurin MV, Livshits VA (1994) Some practical aspects of bacterial electrotransformation. A review // Biologicheskiye Membrany (Russ.) 11 (2): 117 – 139
41.        Tyurin MV, Livshits V. (1993) Bacterial electrotransformation: problems and prospects. A review // Uspekhi Sovremennoj Biologii (Russ.) 113 (6): 659 – 674
42.        Konstantinova NIu, Tiurin MV, Voropaeva EA, Shenderov BA. (1994) [Bacteria of the genus
Eubacterium and their participation in steroid metabolism]. Zh Mikrobiol
Epidemiol Immunobiol (2):114-119. Review
43.        Tiurin MV (1992) Bacterial electrotransformation. Antibiot Khimioter. 37(5):51–55.
44.        Tyurin MV. (1992)
Russian Patent #2005776.
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